| 货号 | AF5999-SP |
| 别名 | CCN1CCNAcyclin-A; CCNA; CCNA2; cyclin A2; Cyclin-A; cyclin-A2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human and mouse Cyclin A2 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 890 (Human); 12428 (Mouse); 114494 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cyclin A2 Ala73-Tyr199 Accession # P20248 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cyclin A2 (also CCNA2) is a member of the cyclin AB subfamily, cyclin family of proteins. Cyclin A2 is ubiquitously expressed, in contrast to CyclinA1 that only appears in late pachytene spermatocytes. It associates with CDK1 during G2 and M phase, and CDK2 in S phase, of the cell cycle. Binding to SCAPER restricts Cyclin A2 to the cytoplasm. In both compartments, it provides substrate specificity to a phosphorylating complex that promotes entry into, and progression through, mitosis. Human Cyclin A2 is 432 amino acids (aa) in length. It contains two cyclin box folds (aa 210‑299 and 309‑430) and four acetylation sites that induce molecule turnover (Lys54/68/95/112). Over aa 73‑199, human Cyclin A2 shares 83% aa identity with mouse Cyclin A2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Cyclin A2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
