| 货号 | AF5977-SP |
| 别名 | AIPP1; ATPase-interacting PDZ protein; PDZ domain containing 11; PDZ domain-containing protein 11; PDZD11; PDZK11; PISP | 全称 | PDZ Domain Containing 11 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human PDZD11 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human PDZD2 and recombinant mouse PDZD2 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51248 (Human); 72621 (Mouse); 302422 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PDZD11 Met1-His140 Accession # Q5EBL8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PDZD11 (PDZ domain-containing protein 11; also AIPP1a and PISP) is a 17 kDa cytosolic protein that bears resemblance to members of the MALS/VELIS family of proteins. It is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. Human PDZD11 is 140 amino acids (aa) in length. It contains but one PDZ domain (aa 47-129) that apparently interacts with the C-terminus of partner proteins. One alternative start site exists that is 32 aa upstream of the standard site. This generates the AIPP1b isoform. Full-length human PDZD11 shares 97% aa identity with mouse PDZD11. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human PDZD11 by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
