| 货号 | AF5976-SP |
| 别名 | 85 kDa nucleoporin; FLJ12549; FROUNT; nuclear pore complex protein Nup85; nucleoporin 85kDa; Nucleoporin Nup75; Nucleoporin Nup85; Nup75; NUP85; PCNT; PCNT1; Pericentrin-1 | 全称 | Nuclear Pore Complex Protein Nup85 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NUP85 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NUP85 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79902 (Human); 445007 (Mouse); 287830 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NUP85 Accession # Q9BW27 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NUP85 (Nucleoporin 85; also Nup75/nucleoporin 75 and FROUNT) is a 70‑72 kDa member of the nucleoporin Nup85 family of proteins. It is one of nine members of the Nup107-160 subcomplex that performs at least two functions in the cell. First, it serves as an assembly point for nuclear pore formation, and may stabilize the bend that normally exists between the inner and outer nuclear membranes. Second, it appears to be quite stable, and during mitosis, promotes mitotic spindle assembly. Human NUP85 is 656 amino acids (aa) in length. It is an alpha -helical protein with no obvious domain(s) or structural motif. Phosphorylation on Ser233, however, may regulate the overall interaction of the NUP107‑160 subcomplex with the much larger nuclear pore. There one potential splice form that shows an alternative start site at Met47. Full-length NUP85 shares 92% aa identity with mouse NUP85. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Nucleoporin NUP85 in Human MSCs. Nucleoporin NUP85 was detected in immersion fixed human multipotent stromal cells (MSCs) using Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Nucleoporin NUP85 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
