| 货号 | AF5959-SP |
| 别名 | EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.4; MGC13356; ST1B1; ST1B2; ST1B2sulfotransferase family cytosolic 1B member 1; Sulfotransferase 1B1; Sulfotransferase 1B2; sulfotransferase family, cytosolic, 1B, member 1; SULT1B1; SULT1B2; Thyroid hormone sulfotransferase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Cytosolic Sulfotransferase 1B1/SULT1B1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) SULT1A1 and rhSULT1A3 is observed and less than 2% cross-reactivity with rhSULT2A1, rhSULT4A1, rhSULT2B1, and rhSULT1E1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 1B1/SULT1B1 (8.33 µg/mL, Catalog # 5959-ST) transfer from the sulfate donor Adenosine 3-phosphate 5-phosphosulfate (PAPS, 33 μM) to 1-Napthol (333 μM). The Neutralization Dose (ND50) is typically 110 µg/mL. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27284 (Human); 56362 (Mouse); 64305 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cytosolic Sulfotransferase 1B1/SULT1B1 aa2-296 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols. Human SULT1B1 shows 72.3% amino acid sequence identity to mouse SULT1B1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytosolic Sulfotransferase 1B1/SULT1B1 by Western Blot. Western blot shows lysates of human CD4+ T Cells and human liver tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1B1/ |
