| 货号 | AF5950-SP |
| 别名 | CGI151; CGI-151; FLJ22190; FLJ38193; KIAA0992FLJ39139; MYN; myoneurin; palladin; palladin, cytoskeletal associated protein; PALLD; PNCA1; Sarcoma antigen NY-SAR-77; SIH002; SIH002FLJ61376 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Palladin in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23022 (Human); 72333 (Mouse); 364558 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Palladin Met1154-Gly1304 Accession # Q8WX93 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Palladin (a cytoarchitectural protein named after the 16th century Venetian architect Palladio) is a cytoplasmic member of the myotilin/palladin family of proteins. Palladin is widely expressed and interacts with actin-associated proteins, thus promoting actin bundle stability. Human Palladin is 1383 amino acids (aa) in length and contains five I‑type Ig-like domains (aa 271-360; 440-539; 1001-1085; 1135-1226; 1233-1324), a Pro-rich region (aa 634-864) and nine potential Ser/Thr phosphorylation sites. Multiple isoform variants exist. Predominant are a 140 kDa and 92-95 kDa short form that show alternative start sites at Met383 and Met712, respectively. The 140 kDa isoform also shows a deletion of aa 656-879 to generate an approximately 115 kDa isoform. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Palladin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, PC‑3 human prostate cancer cell line, and Caki‑2 human clear cell carcinoma epithelial cell line. PVDF Membrane was probed with 0.5 µg/mL of Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Palladin at approximately 95, 115, and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Palladin in Human Prostate. Palladin was detected in immersion fixed paraffin-embedded sections of human prostate using Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
