| 货号 | AF5948-SP |
| 别名 | CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7 | 全称 | LDL Receptor-related Protein 4 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and rat LRP-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-1B, rhLRP-5, rhLRP-6, and rhLDL R is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4038 (Human); 228357 (Mouse); 83469 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LRP-4 Gly18-Ser344 Accession # O75096 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa identity with mouse LRP‑4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat LRP‑4 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and rat cortical embryonic neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat LRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRP‑4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
LRP‑4 in Human Brainstem. LRP‑4 was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human/Rat LRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. This application has not been tested in rat samples. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
