| 货号 | AF5947-SP |
| 别名 | E(Sp1) homolog; enhancer of split groucho 1; ESG; ESG1Enhancer of split groucho-like protein 1; GRG1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer protein 1 | 全称 | Transducin-like Enhancer of Split 1 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7088 (Human); 21885 (Mouse); 362533 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TLE1 His11-Asn200 Accession # Q04724 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln‑rich region that mediates oligomerization (aa 1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa 200-268), and six WD repeats that mediate protein‑protein interaction (aa 470-767). Phosphorylation on Ser will generate 90-93 kDa and 116 kDa forms in SDS‑Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10 aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa 11-200, human TLE1 shares 98% aa identity with mouse TLE1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TLE1 by Western Blot. Western blot shows lysates of human muscle tissue and human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human TLE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TLE1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
