| 货号 | AF5940-SP |
| 别名 | hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; REG3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III | 全称 | Regenerating Islet-derived IIIa |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Reg3A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Reg3G, recombinant mouse (rm) Reg3A, rmReg3B and recombinant rat Reg2 is observed and less than 2% cross-reactivity with rhReg1A, rhReg1B, rmRegIV, rmReg2 and rmReg3D. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5068 (Human); 19694 (Mouse); 171162 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Reg3A Glu27-Asp175 Accession # Q06141 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Reg3A (regenerating gene III alpha ) is a secreted, 17 kDa, type III member of the Reg family, C-type lectin superfamily of proteins. It is expressed in intestine and exocrine pancreas, where it may have a protective effect against infection and TNF-alpha -induced stress. Mature mouse Reg3A is 149 amino acids (aa) in length (aa 27‑175). It is essentially a soluble C-type lectin domain (aa 40‑173). There are four type III genes ( alpha -δ) in the mouse Reg family. Reg3A has less that 60% aa identity to beta -δ. Mature mouse Reg3A is 87% and 61% aa identical to rat and human Reg3A, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Reg3A by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human Reg3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5940) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
