| 货号 | AF5937-SP |
| 别名 | REPA2; Replication factor A protein 2; replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit; replication protein A2 (32kD); replication protein A2, 32kDa; RF-A protein 2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34 | 全称 | Replication Protein A2, 32kDa |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human RPA2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6118 (Human); 19891 (Mouse); 59102 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RPA2 Leu141-Glu270 Accession # P15927 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human RPA2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos‑2 human osteosarcoma cell line, SH‑SY5Y human neuroblastoma cell line, and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RPA2 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
