| 货号 | AF5927-SP |
| 别名 | EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; fatty acid synthase; MGC14367; MGC15706; OA-519; SDR27X1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Fatty Acid Synthase/FASN in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2194 (Human); 14104 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Fatty Acid Synthase/FASN Ser10-Cys212 Accession # P49327 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fatty Acid Synthase; also FAS or FASN) is a cytosolic member of the short chain dehydrogenase/reductase family 27X. It is found as an antiparallel homodimer in neurons, skeletal muscle, adipocytes and hepatocytes. FASN catalyzes the formation of palmitic acid from acetylCoA or malonylCoA, and also likely mediates the transfer of fatty acids to peptides. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fatty Acid Synthase/FASN at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
