| 货号 | AF5918-SP |
| 别名 | HOS; T-box 5; T-box protein 5; T-box transcription factor TBX5 | 全称 | T-box Transcription Factor 5 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Simple Western(50 µg/mL) |
| 目标/特异性 | Detects human and mouse TBX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross‑reactivity with recombinant human (rh) TBX2 is observed and less than 1% cross‑reactivity with rhTBX3, rhTBX6, rhTBX18, and rhTBX20 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 50 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6910 (Human); 21388 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TBX5 Lys253-Lys327 Accession # Q99593 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TBX5 (T-box protein 5) is a 64 kDa member of the T-box family of transcriptional regulators. It is expressed in both embryo and adult, and is particularly important in the development of the heart and upper limb. TBX5 is a transcriptional activator and influences the expression of FGF‑10 and NPPA/ANF. Human TBX5 is 518 amino acids (aa) in length. It contains one T‑box DNA‑binding domain (aa 58‑238), a transactivation motif (Glu349GluAsp351), and an NLS (aa 339‑379). There are multiple splice forms. One 35 kDa isoform shows a RPLL substitution for aa 252‑518, generating a TBX5 antagonist. Other isoforms show an alternative start site at Met51, a 136 aa substitution for aa 445‑518, a 33 aa substitution for aa 360‑518, a 22 aa substitution for aa 328‑518, and a 41 aa substitution for aa 7‑49. Over aa 253‑327, human TBX5 shares 95% aa identity with mouse TBX5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse TBX5 by Western Blot. Western blot shows lysates of human heart and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TBX5 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human and Mouse TBX5 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for TBX5 at approximately 56 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
