| 货号 | AF5900-SP |
| 别名 | FHL5; Hunc18b; MUNC18-2; Protein unc-18 homolog 2; Protein unc-18 homolog B; STXBP2; syntaxin binding protein 2; syntaxin-binding protein 2; Unc18-2; UNC18B; unc-18B; UNC18Bpp10122 | 全称 | Syntaxin-binding Protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Syntaxin‑BP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Syntaxin-BP1 and rhSyntaxin-BP3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6813 (Human); 20911 (Mouse); 81804 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin‑BP2 Glu81-Thr223 Accession # NP_001120868 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntaxin‑BP2 (STXBP2; Syntaxin-binding protein 2; also Unc18-2 and Unc18B) is a 64‑66 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2, and 3. Thus, it presumably participates in primary granule exocytosis by promoting granule fusion. Human STXBP2 is 593 amino acids (aa) in length. It contains one SEC1 domain (aa 28‑576) with Trp28, Ser42, and Glu59 all playing a key role in syntaxin binding. There is one splice variant that shows a deletion of aa 83‑85. Over aa 80‑223, human and mouse STXBP2 share 95% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Syntaxin-BP2 by Western Blot. Western blot shows lysates of human peripheral blood leukocyte. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin-BP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5900) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
