| 货号 | AF5899-SP |
| 别名 | cadherin-like 1; Cadherin-like protein 1; FLJ53887; MGC45991; PC42; PC42PCDH42; PCDH1; PCDH42; protocadherin 1 (cadherin-like 1); protocadherin 1; protocadherin 42; protocadherin-1; protocadherin-42 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Protocadherin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Protocadherin-8 and rhProtocadherin-10 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5097 (Human); 75599 (Mouse); 307481 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Protocadherin‑1 Thr58-Asn162 Accession # Q08174 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protocadherin-1 (PCDH-1; also PC42) is a 150 - 170 kDa δ1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca++-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58 - 852) plus a 364 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58 - 844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There are multiple splice variants. Alternative start sites exist at Met23 and Met235, there is a deletion of aa 209 - 220, and a 27 aa substitution for aa 1034 - 1237. Over aa 58 - 162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Protocadherin-1 in BG01V Human Stem Cells. Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Protocadherin-1 by Western Blot. Western blot shows lysates of human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
