| 货号 | AF5888-SP |
| 别名 | IDDM20; LNKSH2B adapter protein 3; Lymphocyte adapter protein; Lymphocyte-specific adapter protein Lnk; SH2B adaptor protein 3; SH2B3; Signal transduction protein Lnk | 全称 | Lymphocyte Adapter Protein |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human LNK/SH2B3 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10019 (Human); 16923 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. LNK (SH2B3): paradoxical effects in ovarian cancer. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LNK/SH2B3 Gln427-Leu575 Accession # Q9UQQ2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LNK/SH2B3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, Ramos human Burkitts lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
