| 货号 | AF5887-SP |
| 别名 | BMX non-receptor tyrosine kinase; Bone marrow tyrosine kinase gene in chromosome X protein; EC 2.7.10; EC 2.7.10.2; Epithelial and endothelial tyrosine kinase; ETK; Etk/Bmx cytosolic tyrosine kinase; ETKcytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; NTK38; PSCTK2; PSCTK3 | 全称 | BMX Non-receptor Tyrosine Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human BMX in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 660 (Human); 12169 (Mouse); 367786 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BMX Met1-Gln215 Accession # P51813 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BMX (Bone marrow tyrosine kinase gene in chromosome X protein; also ETK and NTK38) is a 76‑85 kDa member of the TEC subfamily, Tyr protein kinase family of enzymes. It is a cytoplasmic endothelial and epithelial cell kinase that induces STAT phosphorylation in a Jak-independent manner, and regulates cytokine production via mRNA stabilization. Human BMX is 675 amino acids (aa) in length. It contains one PH domain whose ligation induces activation (aa 6‑109), a zinc-finger region (aa 113‑149), two autophosphorylation sites at Tyr216 and Tyr224, an SH3 and SH2 domain (aa 213‑392) and a kinase catalytic site (aa 417‑675). BMX undergoes proteolysis by caspase 3 to generate a 35 kDa N-terminal (aa 1‑242) fragment, and a 50 kDa C-terminal (aa 243‑675) fragment with enhanced kinase activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human BMX by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMX at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
BMX in Human Prostate. BMX was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
