| 货号 | AF5879-SP |
| 别名 | ATP-dependent helicase RENT1; EC 3.6.1; EC 3.6.4.-; FLJ43809; HUPF1; KIAA0221FLJ46894; Nonsense mRNA reducing factor 1; NORF1; NORF1delta helicase; pNORF1; regulator of nonsense transcripts 1; RENT1UP Frameshift 1; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; yeast Upf1p homolog | 全称 | Regulator of Nonsense Transcripts 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human RENT1/UPF1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5976 (Human); 19704 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RENT1/UPF1 Asp351-Arg600 Accession # NP_002902 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | UPF1 (Up-frameshift mutation-1; also RENT1, SMG-2 and NORF1) is a 125-130 kDa member of the DNA2/NAM7 helicase family of molecules. It is ubiquitously expressed, and promotes the turnover of mRNA containing premature stop codons. UPF1 is a cytoplasmic factor that contributes to the formation of a UPF complex. During the translation of properly transcribed mRNA, unphosphorylated UPF1 is blocked from interacting with eRF3, a translation initiation factor. In the presence of an improper stop codon, a detection complex termed EJC initiates phosphorylation of UPF1, allowing it to bind to eRF3 and repress translation. Human UPF1 is 1129 amino acids (aa) in length. It contains one C2H2-type Zn finger motif (aa 131-159), an NTPase domain (aa 486-545) and three phosphorylation sites at Ser1107/1127/1129. There are two potential isoforms that show deletions of aa 54-58 and 353-363, respectively. Over aa 351-600, human UPF1 shares 99% aa identity with mouse UPF1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human RENT1/UPF1 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RENT1/UPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RENT1/UPF1 at approximately 125-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
