| 货号 | AF5834-SP |
| 别名 | NFAT4; NF-AT4; NFAT4nuclear factor of activated T-cells, cytoplasmic 3; NFATc3; NF-ATc3; NFATX; nuclear factor of activated T-cells c3 isoform IE-Xa; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4 | 全称 | Nuclear Factor of Activated T Cells C3 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat NFATC3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4775 (Human); 18021 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NFATC3 Asp900-Asp1035 Accession # Q12968 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145‑150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109‑114), two nuclear localization sites (aa 273‑275 and 686‑688), one nuclear export site (aa 1032‑1041) and an RH domain that binds DNA (aa 433‑592). There are multiple isoforms with different tissue expression profiles. Over aa 900‑1035, human NFATC3 shares 93% aa identity with mouse NFATC3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat NFATC3 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat NFATC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC3 at approximately 145 - 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
