| 货号 | AF5828-SP |
| 别名 | alcohol/hydroxysteroid sulfotransferase; Dehydroepiandrosterone sulfotransferase; DHEAS; DHEA-ST; DHEA-STbile salt sulfotransferase; EC 2.8.2.14; HST; hSTa; Hydroxysteroid Sulfotransferase; ST2; ST2A1; ST2A3; STDbile-salt sulfotranasferase 2A1; Sulfotransferase 2A1; sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone(DHEA)-preferring, member 1; SULT2A1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, and rhSULT4A1 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 2A1/SULT2A1 (8.33 µg/mL, Catalog # 5828-ST) transfer from the sulfate donor Adenosine 3-phosphate 5-phosphosulfate (PAPS, 33 μM) to Trans-Dehydroandrosterone (DHEA, 133 µM). The Neutralization Dose (ND50) is typically 35.9 µg/mL. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6822 (Human); 20859 (Mouse); 24912 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1 Ser2-Glu285 Accession # Q06520 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line. Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
