| 货号 | AF5826-SP |
| 别名 | Brain sulfotransferase-like protein; BRSTL1; BR-STL-1; EC 2.8.2.-; hBR-STL; hBR-STL-1MGC40032; nervous system cytosolic sulfotransferase; Nervous system sulfotransferase; NST; ST4A1; sulfotransferase 4A1; sulfotransferase family 4A, member 1; sulfotransferase-related protein; SULT4A1; SULTX3; SULTX3DJ388M5.3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human SULT4A1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT2A1, and rhSULT1E1. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25830 (Human); 29859 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SULT4A1 isoform 1 Ala2-Leu284 Accession # Q9BR01 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cytosolic SULT4A1 (Sulfotransferase 4A1 also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284 amino acids (aa) in length. A weak PAPS binding site is found between aa 246-254. There are two potential isoforms, one that shows a 13 aa substitution for aa 248-284, and a second that contains a 48 aa substitution for aa 202-247. Full length human SULT4A1 is 98% aa identical to mouse SULT4A1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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SULT4A1 in Human Brain SULT4A1 was detected in paraffin-embedded sections of human brain (cortex) using Human SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
