Human/Mouse CX3CR1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse CX3CR1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF5825-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5825-SP
别名	Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1
全称	CX3C Chemokine Receptor 1
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1524 (Human); 13051 (Mouse); 171056 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP Diabetes, 2011;60(5):1512-8. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CX3CR1 Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274 Accession # Q9Z0D9
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CX3CR1 (CX₃C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1^lo monocytes plus smooth muscle and mast cells. CX₃CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX₃CR1 shares 96% and 77% aa identity with rat and human CX₃CR1, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Chemokine Nomenclature Chemokine Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Conventional/Classical Dendritic Cells Glomerulonephritis: Beyond Inflammation Mini-review: Chemokines III Monocyte Markers Mouse Tissue-Resident Macrophage Markers Neutrophils Renal Markers T Cell Migration/Adhesion

参考图片

Detection of Human/Mouse CX3CR1 by Western Blot. Western blot shows lysates of mouse embryonic heart tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CX3CR1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).