| 货号 | AF5790-SP |
| 别名 | CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CD164 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD164 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8763 (Human); 53599 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD164 extracellular domain Asp24-Leu197 Accession # Q04900 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human CD164 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2 |
