| 货号 | AF5786-SP |
| 别名 | forkhead box J3; forkhead box protein J3; KIAA1041; MGC165036; MGC176686 | 全称 | Forkhead Box J3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse FoxJ3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22887 (Human); 230700 (Mouse); 313554 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FoxJ3 Met375-Tyr552 Accession # NP_055762 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FoxJ3 (Forkhead box protein J3) is a 69 kDa (predicted) nuclear protein that is a Class 1 member of the Fox family of transcription factors. It is expressed in embryonic neuroectoderm, neural crest and somitic condensations, and is suggested to participate in terminal tissue differentiation. Human FoxJ3 is 622 amino acids (aa) in length. It contains one forkhead DNA binding region (aa 77‑168) plus a phosphorylation site at Ser223. There are two potential splice variants. One shows a deletion of aa 177‑210, while a second shows a 12 aa substitution for aa 177‑622. Over aa 375‑552, human FoxJ3 shares 93% aa identity with mouse FoxJ3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FoxJ3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse FoxJ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5786) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxJ3 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
