| 货号 | AF5785-SP |
| 别名 | ESG3; ESG3Enhancer of split groucho-like protein 3; ESGenhancer of split groucho 3; Grg3; HsT18976; KIAA1547FLJ39460; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1); transducin-like enhancer protein 3 | 全称 | Transducin-like Enhancer of Split 3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human TLE3 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7090 (Human); 21887 (Mouse); 84424 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TLE3 His11-Asn200 Accession # Q04726 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TLE3 (Transducin-Like Enhancer of Split 3; also ESG3 and GRG3) is an 84‑86 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed in the embryo, and in differentiating epithelial cells in the adult. TLE3 binds to a number of transcription factors as a heterooligomer, or to itself as a homotetramer. Oligomerization is essential for activity. Human TLE3is 772 amino acids (aa) in length. It contains a Gln(Q)-rich region that mediates oligomerization (aa 1‑131), a CCN domain that contains an NLS plus CKII and cdc2K kinase site (aa 199‑268), and seven WD repeats that mediate protein-protein interaction (aa 484‑771). Potentially there are multiple splice variants. Over aa 11‑200, human TLE3shares 99% aa identity wih mouse TLE3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TLE3 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse TLE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TLE3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
