| 货号 | AF5770-SP |
| 别名 | CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein | 全称 | Mannose Receptor, C-type 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Mrc2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse Mrc2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9902 (Human); 17534 (Mouse); 498011 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Mrc2 Cys800-Ala1059 Accession # Q9UBG0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mrc2 (C-type Mannose Receptor 2; also MMR2, Endocytic Receptor 180 and uPARAP) is a 180‑200 kDa member of the macrophage mannose receptor family of proteins. It is widely expressed, being found on fibroblasts, monocytes/macrophages, endothelial cells, keratinocytes and myoepithelial cells. It binds the carbohydrates Man, Fuc and NAcGlc, type V collagen, and pro‑uPA. Mature human Mrc2 is a 1449 amino acid (aa) type I transmembrane protein. It contains a 1384 aa extracellular region and a 44 aa cytoplasmic domain. The extracellular region shows one ricin B‑type lectin domain (aa 41‑167), one fibronectin type II domain (aa 182‑230) and eight C‑type lectin domains (aa 244‑1393). Over aa 800‑1059, human Mrc2 shares 87% aa identity with mouse Mrc2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Mrc2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mrc2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Mrc2 in Human Prostate Cancer Tissue. Mrc2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
