| 货号 | AF5768-SP |
| 别名 | alpha 1,6 Fucosyltransferase; alpha-(1,6)-fucosyltransferase; alpha1-6FucT; EC 2.4.1.68; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); Fucosyltransferase 8; FUT8; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Fucosyltransferase 8/FUT8 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2530 (Human); 53618 (Mouse); 432392 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8 Asp32-Lys575 Accession # Q9BYC5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60‑65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1‑9) plus an extended luminal domain (aa 31‑575) that possesses one SH3 homology domain (aa 502‑563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1‑420, a 32 aa substitution for aa 1‑161, and a two aa substitution for aa 444‑575. Over aa 32‑575, human FUT8 shares 97% aa identity with mouse FUT8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Fucosyltransferase 8/FUT8 in Human Colon. Fucosyltransferase 8/FUT8 was detected in paraffin-embedded sections of human colon using Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Fucosyltransferase 8/FUT8 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 60 - 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human Fucosyltransferase 8/FUT8 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
