| 货号 | AF5766-SP |
| 别名 | RTN4C | 全称 | Reticulon 4C |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects Nogo-C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Nogo-A and rhNogo-B is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57142 (Human); 68585 (Mouse); 83765 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | 11 aa unique to the N-terminus of human Nogo-C synthetic peptide |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Nogo belongs to the reticulon family of transmembrane proteins, which lack an N-terminal signal sequence and possess a 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif. They show punctate intracellular distribution within the ER that is reminescent of a reticulum. The five Nogo isoforms vary from 199 (Nogo-C) to 1192 (Nogo-A; also called reticulon-4) aa in humans. Nogo-B or -C may form complexes with Nogo-A, and all may be expressed in neurons, endothelial cells, oligodendrocytes, fibroblasts and myoblasts. Of 11 aa unique to the N-terminus of human Nogo-C, 9 and 10 aa are identical in mouse and rat Nogo-C, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Nogo‑C by Western Blot. Western blot shows lysates of human skeletal muscle. PVDF Membrane was probed with 1 µg/mL of Human Nogo‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo‑C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Nogo‑C in Human Brain. Nogo‑C was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Human Nogo‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
