| 货号 | AF5740-SP |
| 别名 | cellular retinoic acid binding protein 2; Cellular retinoic acid-binding protein II; CRABP-II; CRABP-IIcellular retinoic acid-binding protein 2; RBP6 | 全称 | Cellular Retinoic Acid Binding Protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human CRABP2 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1382 (Human); 12904 (Mouse); 29563 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activation of the CRABPII/RAR pathway by curcumin induces retinoic acid mediated apoptosis in retinoic acid resistant breast cancer cells | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CRABP2 Met1-Glu138 Accession # P29373 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14 kDa cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138 amino acids (aa) in length and contains one NLS (aa 21‑31) and a series of beta -strands forming a beta -barrel that accommodates retinoic acid in its interior (aa 41‑135). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human CRABP2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, MDA‑MB‑468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
