| 货号 | AF5738-SP |
| 别名 | ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; BRG1-associated factor 190A; BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4; BRM/SWI2-related gene 1; EC 3.6.1; EC 3.6.4.-; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; RTPS2; SMARCA4; SNF2; SNF2B; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; subfamily a, member 4; sucrose nonfermenting-like 4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI2; transcription activator BRG1 | 全称 | BRM/SWI2-related gene 1 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Brg1 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6597 (Human); 20586 (Mouse); 171379 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Brg1 Gln673-Asn774 Accession # P51532 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Brg1 (Brm/Swi2-related gene 1), also known as SMARCA4 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 4), SNF2L4 and SNF2 beta, is a 205 kDa member of the SNF2 helicase family of molecules. It is a ubiquitously expressed, nuclear-localized chromatin remodeling ATPase that, depending upon its associated complex, may both facilitate and inhibit gene transcription. Human SMARCA4 is 1647 amino acids (aa) in length, and contains an HSA domain (aa 460‑532), one BRK region (aa 610‑654), six potential phosphoserine sites between aa 610‑662, a DEXDc domain that unwinds RNA and DNA (aa 774‑913), one Bromo domain (aa 1477‑1547) and eight potential phosphoserine sites between aa 1570‑1644. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Brg1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Brg1 was detected at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Brg1 in HeLa Human Cell Line. Brg1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Brg1 in Rat Cortical Stem Cells. Brg1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
