| 货号 | AF5719-SP |
| 别名 | EC 1.8.1.8; NRN; NRX; NRXFLJ12614; nucleoredoxin 1; nucleoredoxin; NXN; TRG-4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Nucleoredoxin in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64359 (Human); 18230 (Mouse); 360577 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NRX Asn131-Ile435 Accession # Q6DKJ4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Nucleoredoxin (NRX; also known as NXN and Red-1) is a 51‑55 kDa member of the thioredoxin (TXR) family of proteins. It is widely expressed, and exists in both the nucleus and cytoplasm of cells where it regulates two pathways. In the nucleus, NRX may act as a transcription factor in the Wnt-pathway; a stable (reduced) NRX binds to DVL, thereby blocking downstream Wnt/ beta -catenin signaling. It also binds to PP2A subunits PP2AC and PP2AA, generating an active PP2A complex. Human NRX is 435 amino acids (aa) in length and contains a TXR domain (aa 167‑321) that shows a WCPPC catalytic site, and a substrate recognition PDI-b’ region (aa 312‑427). There are three potential isoform variants. Over amino acids 131‑435, human NRX shares 99% aa identity with mouse NRX. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Nucleoredoxin by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Nucleoredoxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleoredoxin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
