| 货号 | AF5717-SP |
| 别名 | BCR downstream-signaling protein 1; BRDG1STAP-1BCR downstream signaling 1; Docking protein BRDG1; signal transducing adaptor family member 1; signal-transducing adaptor protein 1; signal-transducing adaptor protein-1; STAP1; Stem cell adaptor protein 1 | 全称 | BCR Downstream-signaling Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human BRDG1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26228 (Human); 56792 (Mouse); 305269 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BRDG1 Ala177-Ser280 Accession # Q9ULZ2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BCR downstream-signaling protein 1 (BRDG1; also known as Signal-transducing adaptor protein 1, STAP1) is a 36‑37 kDa adaptor molecule that is involved in inflammation. It is expressed in hematopoietic cells, including B cells, macrophages and microglia. BRDG1 is believed to promote and prolong the immune response by controlling cell migration, secretion and phagocytosis. It does so by likely controlling the recruitment of signaling kinases to TRKs, and potentiating BCR-mediated activation of CREB and Elk‑1. Human BRDG1 is 295 amino acids (aa) in length and contains a plextrin homology domain that binds to membrane phospholipids (aa 25‑121), and one SH2 domain that recognizes phosphorylated Tyr residues (aa 176‑259). There is a Tec-mediated phosphorylation site at Tyr65. Over amino acids 177‑280, human BRDG1 shares 82% aa identity with mouse BRDG1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human BRDG1 by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human BRDG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRDG1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
