| 货号 | AF5707-SP |
| 别名 | FABP-9; Fatty Acid Binding Protein 9, Testis; Fatty Acid-Binding Protein 9; Lipid-Binding Protein; PERF 15; PERF; PERF15; Testis Lipid Binding Protein; Testis Lipid-Binding Protein; Testis-Type Fatty Acid-Binding Protein; T-FABP; TLBP | 全称 | Fatty Acid-Binding Protein 9 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP9 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse FABP9 and less than 1% cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 6, 7, and 8 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 646480 (Human); 21884 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP9 Met1-Val132 Accession # Q0Z7S8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP9 (fatty acid binding protein-9; also T-FABP and PERF15) is a cytosolic 15 kDa member of the fatty acid binding protein family, calycin superfamily of proteins. It is found in midpachytene spermatocytes and round spermatids, and constitutes part of the perinuclear theca. Functionally, FABP9 is likely to link intracellular membranes, and may signal abnormal sperm formation during spermatogenesis. Human FABP9 is 132 amino acids (aa) in length and exhibits two layers of antiparallel beta -strands that envelope a hydrophobic pocket for lipid binding. Human FABP9 shares 71% aa identity with mouse FABP9. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP9/T‑FABP in Human Testis. FABP9/T‑FABP was detected in immersion fixed paraffin-embedded sections of human testis using Human FABP9/T‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5707) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
