| 货号 | AF5699-SP |
| 别名 | C11orf32; chromosome 11 open reading frame 32; FLJ21930; FLJ39258; gp250; Low-density lipoprotein receptor relative with 11 ligand-binding repeats; LR11; LR11LDLR relative with 11 ligand-binding repeats; LRP9; mosaic protein LR11; SORL1; SorLA; SorLA-1SORLA; sortilin-related receptor; sortilin-related receptor, L(DLR class) A repeats containing; sortilin-related receptor, L(DLR class) A repeats-containing; Sorting protein-related receptor containing LDLR class A repeats | 全称 | Sortilin-related Receptor Containing LDLR Class A Repeats |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse SorLA in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6653 (Human); 20660 (Mouse); 300652 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prions amplify through degradation of the VPS10P sorting receptor sortilin | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse SorLA Ser82-Asn367 Accession # Q92673 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SorLA (Sorting protein-related receptor containing LDLR class A repeats; also LR11 and SorL1) is a 250-330 kDa, monomeric member of the SORL1 family of proteins. It is expressed on the cell surface (10%) and in Golgi/ER membranes (90%) of neurons and vascular smooth muscle cells. SorLA binds apoE-rich lipoproteins plus the 39 kDa RAP. It also acts as an intracellular sorting receptor for APP. In this context, it blocks transport of APP into compartments that exhibit beta -secretase activity, thus interfering with A beta peptide generation. Human SorLA is a type I transmembrane glycoprotein 2214 amino acids (aa) in length. It contains a 28 aa signal sequence, a 53 aa propeptide, and a 2133 aa mature molecule. The mature molecule contains a 2056 aa extracellular domain (ECD) (aa 82-2137) and a short 56 aa cytoplasmic tail. The ECD is highly modular, containing five BNR/Asp-box repeats (aa 136-573), five LDLR class B repeats (aa 800-1013), one EGF-like domain (aa 1026‑1072), 11 LDLR class A domains (aa 1076-1551) and six FN type III repeats (aa 1554-2023). Multiple potential splice variants exist. There are alternative start sites at Met1057 and Met1155, plus a 63 aa substitution for aa 1-1153 and a 19 aa substitution for aa 1-1404. Over aa 82-367, human SorLA shares 93% aa identity with mouse SorLA. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse SorLA by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorLA at approximately 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
SorLA in Human Brain. SorLA was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
