Human FOLR2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human FOLR2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF5697-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5697-SP
别名	beta-HFR; FBP; FBP/PL-1; folate receptor 2 (fetal); Folate receptor 2; folate receptor beta; folate receptor, beta; Folate receptor, fetal/placental; folate-binding protein, fetal/placental; FR-beta; FR-P3; Placental folate-binding protein
全称	Folate Receptor 2
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human FOLR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2350 (Human); 14276 (Mouse); 293154 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glucocorticoids enhance CD163 expression in placental Hofbauer cells. Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S Endocrinology, 2013;154(1):471-82. Species: Human Sample Type: Cell Lysate Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human FOLR2 Gln22-His228 Accession # P14207
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Fowler, B. et al. (2001) Kidney Int. 59:S-221. Kelemen, L.E. (2006) Int. J. Cancer 119:243. Shen, F. et al. (1994) Biochemistry 33:1209. Ratnam, M. et al. (1989) Biochemistry 28:8249. Ross, J.F. et al. (1999) Cancer 85:348. Reddy, J.A. et al. (1999) Blood 93:3940. Ross, J.F. et al. (1994) Cancer 73:2432. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

参考图片

Detection of Human FOLR2 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR2 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

FOLR2 in Human Neutrophils. FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.