| 货号 | AF5688-SP |
| 别名 | EC 3.4.21; EC 3.4.21.4; Polyserase 1; Polyserase-1; Polyserase-I; Polyserine Protease 1; Transmembrane Protease Serine 9; Transmembrane Protease, Serine 9; Transmembrane Serine Protease 9 | 全称 | Transmembrane protease, serine 9 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TMPRSS9 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 360200 (Human); 432478 (Mouse); 314636 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TMPRSS9 Glu190-Gln490 Accession # Q7Z410 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TMPRSS9 (Transmembrane protease, Serine 9; also Polyserine protease 1) is a 116-120 kDa (predicted) member of the TTSP family of membrane-bound serine proteases. It is expressed in fetal tissue, and converts pro-uPA into its active form. Human TMPRSS9 is a 1059 type II transmembrane (TM) protein. It has a 29 aa N-terminal cytoplasmic region and a 1008 aa extracellular domain (ECD) (aa 51-1059). The ECD contains one LDLR region (aa 153-190), followed by three Peptidase S1 domains (aa 203-436, 504-736, and 827-1058), the first two of which are catalytically active. Proteolytic cleavage can generate a soluble 34 kDa Serase 1 (aa 203-503), a 35 kDa Serase 2 (aa 504-826) and an inactive 25 kDa Serase 3 (aa 827-1059). Alternatively, truncated, TM forms of TMPRSS9 such as a 56 kDa TM isotype containing only the Serase 1 domain, or a 91 kDa (predicted) form containing the Serase 1, 2 and 3 domains may exist. Two potential splice forms are reported. One shows an insertion of 34 aa after Lys90 accompanied by a seven aa substitution for aa 491-1059. A second isoform shows a 34 aa substitution for aa 822-1059. Over aa 190-490, human TMPRSS9 shares 79% aa identity with mouse TMPRSS9. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TMPRSS9 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human TMPRSS9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TMPRSS9 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
