| 货号 | AF5687-SP |
| 别名 | SED5; STX5; STX5ASED5; syntaxin 5; syntaxin 5A; syntaxin-5 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(1-15 µg/mL) |
| 目标/特异性 | Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 1-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6811 (Human); 56389 (Mouse); 65134 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin 5 isoform 2 Met55-Thr300 Accession # Q13190 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntaxin 5 (STX5) is a predicted 41‑42 kDa member of the syntaxin family of proteins. It is a t‑SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis‑Golgi network (a 37 kDa form) or the ER (the 46 kDa form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 355 amino acids (aa) in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t‑SNARE domain (aa 263‑325) with a coiled‑coil region (aa 287‑318), and a C‑terminal transmembrane sequence. The 35 kDa short form exhibits an alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19 aa substitution for aa 303‑355. Over aa 55‑300, human STX5 shares 94% aa identity with mouse STX5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Syntaxin 5 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Syntaxin 5 at approximately 38 and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Syntaxin 5 in Human Brain. Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Syntaxin 5 in SH-SY5Y Human Cell Line. Syntaxin 5 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Syntaxin 5 in Rat Brain. Syntaxin 5 was detected in immersion fixed frozen sections of rat brain using Goat Anti-Human Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 3 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
