| 货号 | AF5675-SP |
| 别名 | EIEE4; FLJ37475; hUNC18; MUNC18-1; NSEC1; N-Sec1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; rbSec1; STXBP1; syntaxin binding protein 1; syntaxin-binding protein 1; UNC18; unc18-1; UNC18A; unc-18A; UNC18neuronal SEC1 | 全称 | Syntaxin-binding Protein 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Syntaxin-BP1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Syntaxin-BP2 and rhSyntaxin-BP3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6812 (Human); 20910 (Mouse); 25558 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin-BP1 Ser80-Ala226 Accession # P61764 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | STXBP1 (Syntaxin-binding protein 1; also p67, Munc18-1, N-Sec1 and Unc18A) is a 67 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is expressed in a variety of cells, presumably in those that exhibit granule exocytosis, and likely participates in (synaptic) granule release. Ligands for STXBP1 include STX1A, 2 and 3, and it is possible that STXBP1 both suppresses STX activity, and serves as an anchor for STX-mediated vesicle fusion. Human STXBP1 is 594 amino acids (aa) in length. It contains one SEC1 domain (aa 41‑575). Phosphorylation at Ser313 may stimulate granule release. There is one splice variant that shows a 28 aa substitution for aa 576‑594. This is restricted to neural tissue. Over aa 80‑226, human and mouse STXBP1 are absolutely identical in amino acid sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Syntaxin-BP1 in Mouse Brain. Syntaxin-BP1 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Human/Mouse/Rat Syntaxin-BP1 by Western Blot. Western blot shows lysates of rat brain tissue and spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP1 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
