| 货号 | AF5664-SP |
| 别名 | STX6; syntaxin 6; syntaxin-6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross‑reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10228 (Human); 58244 (Mouse); 60562 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin 6 Ser69-Asn217 Accession # O43752 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Syntaxin 6 by Western Blot. Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 6 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Syntaxin 6 in Human Brain. Syntaxin 6 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to Golgi vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
