Rat VEGF 164 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat VEGF 164 Affinity Purified Polyclonal Ab (25 UG)

货号： AF564-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF564-SP
别名	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
全称	Vascular Endothelial Growth Factor
反应种属	Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF₁₆₅ and rhVEGF₁₂₁ is observed and less than 2% cross-reactivity with rhVEGF-B, recombinant mouse (rm) VEGF-B, rhVEGF-C, rhVEGF-D, and rmVEGF-D is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF₁₆₄-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 20 ng/mL Recombinant Rat VEGF₁₆₄.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Renal primordia activate kidney regenerative events in a rat model of progressive renal disease. Authors: Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M PLoS ONE, 2015;10(3):e0120235. Species: Rat Sample Type: Whole Tissue Application: IHC Not Specified FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy. Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J PLoS ONE, 2015;10(7):e0134392. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity. Authors: Wang H, Yang Z, Jiang Y, Flannery J, Hammond S, Kafri T, Vemuri S, Jones B, Hartnett M Invest Ophthalmol Vis Sci, 2014;55(2):737-44. Species: Rat Sample Type: In Vivo Application: In vivo Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure. Authors: Westenbrink BD, Ruifrok WP, Voors AA Cardiovasc. Res., 2010;87(1):30-9. Species: Rat Sample Type: In Vivo Application: In vivo Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor. Authors: Irani Y, Brereton HM, Tilton RG Hybridoma (Larchmt), 2009;28(3):205-9. Species: Human Sample Type: recombinant protein Application: Binding Assay VEGF promotes vascular sympathetic innervation. Authors: Marko SB, Damon DH Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52. Species: Rat Sample Type: In Vivo Application: In vivo Neutralizing VEGF decreases tortuosity and alters endothelial cell division orientation in arterioles and veins in a rat model of ROP: relevance to plus disease. Authors: Hartnett ME, Martiniuk D, Byfield G, Geisen P, Zeng G, Bautch VL Invest. Ophthalmol. Vis. Sci., 2008;49(7):3107-14. Species: Rat Sample Type: In Vivo Application: In vivo Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1. Authors: Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY J. Mol. Cell. Cardiol., 2008;45(1):44-55. Species: Mouse Sample Type: In Vivo Application: In vivo Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions. Authors: Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE Proc. Natl. Acad. Sci. U.S.A., 2007;104(36):14448-53. Species: Rat Sample Type: In Vivo Application: In vivo Effects of red wine polyphenols on postischemic neovascularization model in rats: low doses are proangiogenic, high doses anti-angiogenic. Authors: Baron-Menguy C, Bocquet A, Guihot AL, Chappard D, Amiot MJ, Andriantsitohaina R, Loufrani L, Henrion D FASEB J., 2007;21(13):3511-21. Species: Rat Sample Type: In Vivo Application: In vivo Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression. Authors: Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM Mol. Pharmacol., 2007;72(2):440-9. Species: Rat Sample Type: Whole Cells Application: Neut Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81. Species: Rat Sample Type: Whole Cells Application: Neut Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia. Authors: Yeh WL, Lin CJ, Fu WM Mol. Pharmacol., 2007;73(1):170-7. Species: Rat Sample Type: Cell Culture Supernates Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat VEGF₁₆₄ Ala27-Arg190 Accession # AAL07526
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164, and 188 aa isoforms found in rat and mouse. Isoforms other than VEGF₁₂₀ and VEGF₁₂₁ contain basic heparin-binding regions and are not freely diffusible. Rat VEGF₁₆₄ shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF₁₆₅ binds the semaphorin receptor, neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Adult Neurogenesis Airway Inflammation and Remodeling ALS Related Molecules BIObrief: Neuroprotective & Pathogenic Effects of VEGF Signaling Cancer Biomarkers Developmental Factors Implicated in Neurodegeneration HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: VEGF Inflammatory Mediators Macrophage Activation Markers Mini-review: Vascular Endothelial Growth Factor (VEGF) Myeloid-derived Suppressor Cells (MDSC) New and Improved TNF Superfamily Proteins 2015 Platelets Preeclampsia Proliferation Bioassay HUVECs Regulation of VE-Cadherin and VEGF R2 by VEGF Technical Notes: Serum Levels of Vascular Growth Factors During Pregnancy VEGF & Amyotrophic Lateral Sclerosis VEGF Family VEGF Family Ligands VEGF, Angiopoietin-2 and Tumor Vascularization

参考图片

VEGF₁₆₄ in Rat Kidney.VEGF₁₆₄ was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Goat Anti-Rat VEGF₁₆₄ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by VEGF₁₆₄ and Neutralization by Rat VEGF Antibody. Recombinant Rat VEGF₁₆₄(Catalog #564-RV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat VEGF₁₆₄ (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564). The ND₅₀ is typically 0.2-0.6 µg/mL.