| 货号 | AF5648-SP |
| 别名 | MGC90328; STX16; Syn16; SYN16hsyn16; syntaxin 16; syntaxin-16 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Syntaxin 16 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Syntaxin 1A, rhSyntaxin 6, and rhSyntaxin BP1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8675 (Human); 228960 (Mouse); 362283 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin 16 isoform B Leu165-Lys301 Accession # O14662 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntaxin 16 (STX16; also SNY16) is a 39 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325 amino acids (aa) in length. It contains a cytoplasmic syntaxin region (aa 74‑180), a coiled-coil region (aa 230‑292), and a short, three amino acid C‑terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa 45‑48, 28‑48 and 28‑44, respectively. A cytoplasmic form shows an Ala substitution for aa 132‑325. Over aa 165‑301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Syntaxin 16 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 16 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
