| 货号 | AF5645-SP |
| 别名 | EC 3.1.1; EC 3.1.1.1; KIAA1366neuroligin-2; neuroligin 2; NLGN2 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and rat Neuroligin 2/NLGN2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NGLN1, rhNGLN3, and rhNGLN4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57555 (Human); 216856 (Mouse); 117096 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Neuroligin 2/NLGN2 Gln15-Ser660 Accession # EAW90197 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function. Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail. Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD. This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion. The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses. Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3. Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners. Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3. Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2-dependent GABAergic contacts. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot. Western blot shows lysates of human cortex and rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuroligin 2/NLGN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroligin 2/ |
