| 货号 | AF5640-SP |
| 别名 | MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATc1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | 全称 | Nuclear Factor of Activated T Cells C1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human NFATC1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4772 (Human); 18018 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NFATC1 Ala48-Ser406 Accession # O95644 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120 kDa member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Human NFATC1 is 943 amino acids (aa) in length. It contains a calcineurin-binding site (aa 118‑123), two transactivation domains (aa 126‑218 and 703‑943), two NLSs (aa 265‑267 and 682‑684), an NES (aa 310-321) and an RHD that binds DNA (aa 410‑592). There are multiple isoforms. Individually or in common, there is an alternate start site at Met473, a deletion of aa 826‑943, a 29 aa substitution for aa 1‑42, and a 19 aa substitution for aa 698‑943. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human NFATC1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Ramos human Burkitts lymphoma cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC1 at approximately 110 - 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human NFATC1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for NFATC1 at approximately 124 kDa (as indicated) using 10 µg/mL of Goat Anti-Human NFATC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
