| 货号 | AF5635-SP |
| 别名 | Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF 2; TERF2; TRBF 2; TRBF2; TRF 2; TRF2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2 | 全称 | Telomeric Repeat Binding Factor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human TRF2 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7014 (Human); 21750 (Mouse); 361403 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRF2 Gln78-Thr238 Accession # Q15554 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55‑60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. TRF2 has been found up regulated in several human cancers. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13‑30), a dimerization domain (aa 46‑112), an NLS (aa 329‑333), and a DNA binding HTH myb-type domain (aa 442‑499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239‑500. Over aa 78‑238, human TRF2 shares 96% aa identity with mouse TRF2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human TRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5635) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
