| 货号 | AF5634-SP |
| 别名 | activating enhancer binding protein 2 beta; Activating enhancer-binding protein 2-beta; AP-2B; AP2-B; AP2-beta; CHAR; MGC21381; TFAP2B; transcription factor AP-2 beta (activating enhancer binding protein 2 beta); transcription factor AP-2 beta (activating enhancer-binding protein 2 beta); transcription factor AP-2-beta | 全称 | Activating Enhancer-binding Protein 2 beta |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human AP-2 beta in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7021 (Human); 21419 (Mouse); 301285 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AP-2 beta Leu121-Phe218 Accession # Q92481 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human AP‑2 beta by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human AP‑2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5634) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AP‑2 beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
