| 货号 | AF5594-SP |
| 别名 | Ajuba; JUB; jub, ajuba homolog (Xenopus laevis); MGC15563; protein ajuba |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human Ajuba in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84962 (Human); 16475 (Mouse); 85265 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Ajuba Met1-Arg121 Accession # Q96IF1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Ajuba (an Urdu word meaning "curiosity") is a 55‑60 kDa member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha -catenin:F-actin binding, beta -catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kappa B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa 1‑335), an NES (aa 280‑287), and three LIM domains that bind multiple proteins (aa 336‑397, 401‑461 and 462‑530). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Ajuba by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Ajuba Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5594) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ajuba at approximately 55 to 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
