| 货号 | AF5589-SP |
| 别名 | leucine-rich repeat transmembrane neuronal protein 2; leucine rich repeat transmembrane neuronal 2; LRRN2 | 全称 | Leucine Rich Repeat Transmembrane Neuronal 2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse LRRTM2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) LRRTM1, rhLRRTM3, and rhLRRTM4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26045 (Human); 107065 (Mouse); 685472 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Nuclear calcium signaling induces expression of the synaptic organizers Lrrtm1 and Lrrtm2. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human LRRTM2 Cys34-Arg422 Accession # O43300 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59 kDa (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483 amino acids (aa) in length. The extracellular region contains ten LRRs (aa 61‑299), followed by a 73 aa cytoplasmic tail (aa 444‑516). Over aa 34‑422. human LRRTM2 shares 98% aa identity with mouse LRRTM2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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LRRTM2 in Embryonic Mouse Neural Tube. LRRTM2 was detected in immersion fixed frozen sections of embryonic mouse neural tube (E10.5) using 10 µg/mL Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. | |
LRRTM2 in Mouse Embryo. LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Human/Mouse LRRTM2 by Western Blot. Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
LRRTM2 in Mouse Neural Tube. LRRTM2 was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
