| 货号 | AF5588-SP |
| 别名 | HMAT1; HMAT115kD; HUMMAT1H; MAT1H; PEA-15; PED; PED15 kDa phosphoprotein enriched in astrocytes; phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in diabetes | 全称 | Phosphoprotein Enriched in Astrocytes 15 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse PEA-15 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8682 (Human); 18611 (Mouse); 364052 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PEA-15 Ala2-Ala130 Accession # Q15121 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PEA-15 (Phosphoprotein Enriched in Astrocytes of 15 kDa) is a 15 kDa cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130 amino acids (aa) in length. It contains a death effector domain (aa 3‑81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21 aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36‑58. Full-length human and mouse PEA-15 show 99% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PEA‑15 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PEA‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PEA‑15 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
PEA‑15 in Human Brain. PEA‑15 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Mouse PEA‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5588) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
