| 货号 | AF5577-SP |
| 别名 | BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein | 全称 | Mesoderm Development Candidate 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross‑reactivity with recombinant mouse MESDC2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23184 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MESDC2 Ala34-Leu234 Accession # Q14696 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 26 kDa protein (predicted) that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human MESDC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5577) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MESDC2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
