| 货号 | AF5575-SP |
| 别名 | alpha-taxilin; DKFZp451J0118; IL14; IL-14; interleukin 14; MGC118870; MGC118871; RP4-622L5.4; taxilin alpha; TXLN; TXLNA |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human alpha -Taxilin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) gamma ‑Taxilin and rh beta ‑Taxilin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 200081 (Human); 109658 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human alpha -Taxilin Met1-Lys162 Accession # P40222 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | alpha ‑Taxilin (TXLN‑ alpha ; also p75 [in rat] and IL‑14 alpha ) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kDa in SDS‑PAGE. alpha ‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha ‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha ‑Taxilin also regulates the placement of growing protein chains. Human alpha ‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha. Over aa 1‑162, human alpha ‑Taxilin shares 81% aa identity with mouse alpha ‑Taxilin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human alpha -Taxilin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human alpha -Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha -Taxilin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
alpha ‑Taxilin in HepG2 Human Cell Line. alpha ‑Taxilin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human alpha ‑Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
