| 货号 | AF5574-SP |
| 别名 | 6C6-Ag; Bap31,6C6-AG; BAP31DXS1357ECDM; BCAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; BCR-associated protein Bap31,6C6-AG tumor-associated antigen; p28 Bap31,6C6-Ag; p28; Protein CDM | 全称 | B-cell Receptor-associated Protein 31 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human BAP31 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10134 (Human); 27061 (Mouse); 293852 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BAP31 Gly165-Glu246 Accession # P51572 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BAP31 (BCR-associatedprotein of 31 kDa) is a 29 kDa member of the BCAP29/BCAP31 family of proteins. It is ubiquitously expressed, resides in the ER and serves multiple functions. It is known to act as a cargo receptor for ER export of transmembrane (TM) proteins, and serve as a component of the ER quality control compartment, cycling between the peripheral and juxtanuclear ER. Human BAP31 is a 246 amino acid (aa), 3-TM domain containing protein. Amino acids 1‑6 are lumenal, 124‑246 are cytoplasmic, and 7‑27, 44‑64 and 103‑123 constitute TM segments. BAP31 forms homodimers and heterodimers with BAP29. Cleavage between Asp164Gly165 and Asp238Gly239 generates 20 kDa and 27 kDa fragments, respectively. There is an alternate start site 67 aa upstream from the standard start site. Over aa 165‑246, human BAP31 shares 82% aa identity with mouse BAP31. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human BAP31 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BAP31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAP31 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
