| 货号 | AF5559-SP |
| 别名 | C13orf24; chromosome 13 open reading frame 24; KIAA1008; PIBF; progesterone immunomodulatory binding factor 1; progesterone-induced blocking factor 1; progesterone-induced-blocking factor 1; RP11-505F3.1 | 全称 | Progesterone Immunomodulatory Binding Factor 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PIBF1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10464 (Human); 52023 (Mouse); 306104 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PIBF1 Glu19-Ala419 Accession # Q8WXW3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PIBF-1 (Progesterone induced blocking factor 1) is a 90-92 kDa progesterone-inducible molecule initially isolated from the lymphocytes of pregnant women. It is produced by lymphocytes plus villous trophoblast cells, and modulates the activity of cytotoxic NK cells. Human PIBF-1 is 757 amino acids (aa) in length. It contains a possible signal sequence (aa 1-26), two Leu-zippers (aa 311-323 and 643-664), two NLSs (aa 282-285 and 567-573), a bZIP sequence (aa 574-614), and an ER retention motif (aa 752-757). PIBF-1 is found in the nucleus, cytoplasm, and circulation, the result of multiple splice variants. SDS-page shows 90 kDa, 80-83 kDa, 48-52 kDa, 34-35 kDa, and 10-12 kDa isoforms. The 90 kDa form is nuclear and full-length, while the 34-35 kDa form is secreted and likely represents aa 1-223 spliced to aa 683-757. There is a 50-55 kDa form that is apparently intracellular, possibly bioactive and likely represents the N-terminus of the molecule. Over aa 19‑419, human PIBF-1 shares 88% aa identity with mouse PIBF-1.
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| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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PIBF1 in Human Breast Cancer Tissue. PIBF1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human PIBF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5559) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human PIBF1 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and human breast cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PIBF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PIBF1 at approximately 98 kDa and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
